Colony-Forming-Unit (CFU) Assay With High-Resolution Digital Imaging: A Reliable System for Cord Blood (CB) CFU Evaluation
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چکیده
منابع مشابه
Assay of Colony Forming Units in Culture (cfu-c)
The colony forming units in culture (cfu-c) in bone marrowcells, which were suppressed by immunosuppressive factor obtained from the serum of sarcoma 180 tumor bearing mouse, was restored to normal level by the addition of FR-900490in vitro. Furthermore, in mitomycin C (MMC)-treated mice the subsequent administration of FR-900490caused a significant increase of cfu-c in bone marrow cells depres...
متن کاملProspective isolation and global gene expression analysis of the erythrocyte colony-forming unit (CFU-E).
The erythrocyte colony-forming unit (CFU-E) is a rare bone marrow (BM) progenitor that generates erythrocyte colonies in 48 hours. The existence of CFU-Es is based on these colonies, but CFU-Es have not been purified prospectively by phenotype. We have separated the "nonstem," "nonlymphoid" compartment (lineage marker [lin]-c-Kit+Sca-1-IL-7Ralpha-) into interleukin 3 receptor alpha negative (IL...
متن کاملIn situ radiation sensitivity of recombinant human granulocyte colony-stimulating factor-recruited murine circulating blood and bone marrow progenitors (colony-forming unit [CFU]-granulocyte-macrophage and CFU-megakaryocyte): evidence for possible biologic differences between mobilized blood and bone marrow.
Increasing evidence especially stemming from peripheral blood progenitor transplantation studies points to a possible biologic difference between mobilized blood and bone marrow progenitor cells. The objective of this study was to compare the in situ radiation sensitivity of recombinant human granulocyte colony-stimulating factor (rhG-CSF)-recruited circulating granulopoietic (blood colony-form...
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BACKGROUND In vitro colony-forming unit cell (CFU-C) assays are usually-used to detect the quantitative and qualitative features of haematopoietic stem cells (HSCs). We studies CFU-C assays in bone marrow samples from 365 consecutive subjects with newly-diagnosed myelodysplastic syndrome (MDS). Data were interrogated for associations with prognosis. METHODS CFU-C assays were performed accordi...
متن کاملStimulatory effects of granulocyte colony-stimulating factor on colony-forming units-spleen (CFU-S) differentiation and pre-CFU-S proliferation in mice.
Granulocyte colony-stimulating factor (G-CSF) was reported to increase the number of colony-forming units-spleen (CFU-S) and multilineage colonies as well as myeloid-committed cells. We investigated the effects of G-CSF on myeloid progenitors and primitive stem cells in a mouse bone marrow transplantation (BMT) system. Lethally irradiated mice received BM cells from untreated or 5-fluorouracil-...
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ژورنال
عنوان ژورنال: Biology of Blood and Marrow Transplantation
سال: 2009
ISSN: 1083-8791
DOI: 10.1016/j.bbmt.2008.12.137